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KMID : 1102020180480040087
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Applied Microscopy
2018 Volume.48 No. 4 p.87 ~ p.95
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Overview of Immunoelectron Microscopy
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Park Chang-Hyun
Kim Hong-Lim
Chang Byung-joon
Lee Sang-Hoon
Chang Byung-Soo
Bae Chun-Sik
Cho Ik-Hyun
Kim Dong-Heui
Han Jung-Mi
Na Ji-Eun
Choi Byung-Jin
Kim Sang-Sik
Kim Hyun-Wook
Kim Jee-Woong
Rhyu Im-Joo
Uhm Chang-Sub
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Abstract
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Immunoelectron microscopy using an antigen-antibody reaction in an electron microscope is a very useful tool to identify the components of a tissue in an electron microscope. Many researchers also use immunoelectron microscopy. Nonetheless, immunoelectron microscopy is rarely introduced systematically, and immunoelectron microscopy can be carried out without fully understanding the principles, and cases of poor understanding can often be seen in the vicinity. Therefore, in order to make it easier to understand, we will first introduce the principles of immunoelectron microscopy and describe practical methods.
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KEYWORD
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Pre-embedding, Post-embedding, Immuno cytochemistry, Correlative light and electron microscopy
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